Improper use of the hottest natural reagents and i

2022-07-26
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Nature: improper use of reagents and instruments will destroy the experiment. Advanced technology has brought convenience to the experiment, but it has also caused many problems. Two researchers from the Li Ka Shing Center of Cambridge Research Institute in the UK and Vanderbilt University in the United States published their own experiences in the journal Nature. One laboratory recommended inappropriate negative control in the purchased reagents, The previous twelve months' efforts were in vain; Another laboratory misused the commercial automatic imaging analysis system and got the opposite conclusion. These facts tell us that we should not rely on commercial reagents and instruments. Before use, we should understand the composition of reagents and the working principle of instruments

in the first review article, research tools: a recipe for disaster, Anna git of Cambridge Research Institute said in detail that her laboratory encountered the devil of each scientist earlier this year. First, she inserted the twists and turns into each sample, and the 12-month experiment paid off because they found that the chemically synthesized RNA strand (Mi technical name technical parameter crorna MICs) purchased from a biotechnology company, The recommended negative control is not appropriate. This sequence is too short to be resolved. Since the company did not publish more detailed information about this control, they could only find accidental differences by analyzing a variety of known microRNA sequences and observing the length related activity

the worst thing is that the ingredients that have been tested for a long time in the laboratory belong to the confidential content of the manufacturer. Therefore, the ingredients of its chemical substances - enzymes or reagents cannot be published in detail. Without this part, researchers can only chase data and waste time. Experiments are also limited. Some can be done and some cannot be done. The most troublesome thing is that they may unknowingly conduct wrong experiments and publish misleading results

in another review article "research tools: understand how it works with very weak output signals of R sensors", dav3, director of the core Microscopy Laboratory of Vanderbilt University, and ID W. piston described what happened in his colleagues' laboratory. The lab is analyzing protein interactions in cells. After they operated and disrupted the interaction between two special proteins in mitochondria, they still observed this protein interaction. In order to detect this interaction, they used a commercially purchased automatic imaging analysis system. But they did not understand the working principle of the instrument, so they adopted the settings of another colleague in different experiments

however, no other components of the cell were seen in the imaging, only mitochondria appeared. If the researcher modifies the setting to make the whole cell appear, then it can be seen that these proteins appear in the granules in a relatively small range of other parts of the cell, and the interaction between these proteins has been disrupted, which indicates that the previous operation was successful

in this case, what is lacking is not inspiration, but specification. The researcher adopted an experimental tool, but did not carry out appropriate operation. The researcher wasted two months trying to carry out successful experimental operation, but in fact he has succeeded, but he did not see it

unfortunately, this situation is not uncommon. Researchers, especially those who are still in training, do not operate correctly when using experimental equipment, or even automatic tools that have been established in the laboratory. Twenty years ago, scientists needed to program themselves, so they needed to know all the details. For example, when using a microscope, he (or she) needs to understand how this instrument is deployed. However, today, bioscience is full of various experimental tools. Young scientists can get results by simply pressing a button, sending samples, or inserting data. Is this progress or regression

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